Vanadate disrupts mammary gland development in whole organ culture
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چکیده
منابع مشابه
Hormone-inducible casein messenger RNA in a serum-free organ culture of whole mammary gland.
The whole second thoracic mammary gland of estradiol-17beta + progesterone primed 3- to 4-week-old BALB/c female mice was induced to pregnancy-like lobulo-alveolar morphogenesis after 6-day cultivation in a serum-free culture medium containing a "growth promoting" hormone mixture, insulin + prolactin + growth hormone (somatotropin) + estradiol + progesterone. No radioimmunologically detectable ...
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In organ cultures of mammary glands from mice in midpregnancy, addition of both insulin and prolactin induces a marked accumulation of alpha-lactalbumin, whereas the augmentation of casein synthesis requires the presence of insulin, prolactin, and cortisol. Addition of 0.5 mM dibutyryl cyclic AMP resulted in complete inhibition of alpha-lactalbumin accumulation and partial inhibition of casein ...
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Human mammary gland explants obtained (as far from the lesion as possible) from female patients who had undergone surgical biopsy for fibroadenoma of the breast were organ cultured in a chemically defined medium, and their hormone requirement for growth and differentiation was studied. Most of the expiants that were cultured in hormone-free medium survived, while the addition of insulin gave fu...
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In order to improve reliability and standardization of mammary gland whole mount assessments, directors and staff of seven laboratories from around the world (see attached) participated in a round robin evaluation of a set of mammary gland whole mounts. These preparations were from the 4 and 5 mammary glands of mice and rats treated in utero with vehicle and 2 different chemical treatments. Who...
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ژورنال
عنوان ژورنال: Developmental Dynamics
سال: 2001
ISSN: 1058-8388,1097-0177
DOI: 10.1002/dvdy.1193